1. Using the gene recombinant in vitro technique replace the gene of ThyA/illotycin dipl-antibiotic resistance expression vector pW425et to the GFP gene that contain the strong promoter, constructed non-antibiotic resistance tagging expression vector pW425t-GFP, The methods just as extract plasmid, restriction enzyme correspond restriction endonuclease, PCR and so on can be used to construct recombinant masculine cloning plasmid pW425t-GFP. 然后應(yīng)用基因體外重組技術(shù)，將ThyA／紅霉素雙抗性表達(dá)載體pW425et中的紅霉素抗性基因替換為含有強(qiáng)啟動(dòng)子的GFP基因，構(gòu)建了非抗生素抗性的示蹤表達(dá)載體pW425t-GFP，通過(guò)質(zhì)粒提取、限制性內(nèi)切酶酶切、PCR等方法鑒定構(gòu)建出的重組陽(yáng)性表達(dá)載體pW425t-GFP，并通過(guò)SDS-PAGE電泳檢測(cè)綠色熒光蛋白基因的表達(dá)量。
masculine在線翻譯
2. Cao cao`s works showed masculine spirit, strength and a sense of glory and pity at the same time; Cao pi`s, gentleness and beauty; Cao zhi`s, first energetic and valiant, later became melancholy and sad. 曹操的詩(shī)古直悲涼，有一種雄渾壯闊的悲壯美；曹丕的詩(shī)婉約哀怨，是一種柔和秀麗的陰柔美；曹植前期的詩(shī)昂揚(yáng)豪邁，而后期轉(zhuǎn)為抑郁哀傷。
3. For a start todays promise rings are thicker and more masculine looking... 為一個(gè)開(kāi)始，今天的承諾，戒指厚和更男性化的期待。。。
4. The active, masculine cosmic principle in Chinese dualistic philosophy. 正是在這里男性與女性的互補(bǔ)性明顯表現(xiàn)出來(lái)。
5. Poem Duxiu Peak Guilin mountains and rivers less graceful cool lake, more of the the Northern mountains of vigorous and masculine. 詩(shī)中的獨(dú)秀峰少了桂林山水的娟秀優(yōu)柔，多了的到有北方山勢(shì)的雄渾與陽(yáng)剛。
6. We two together stood here wet and trembling, Suddenly, you wraped my waist by the strong masculine hand and stuck in my lips with a feral avidity.... 我們兩個(gè)一起站在這里濕和顫抖，突然，你wraped我的腰圍由強(qiáng)烈的陽(yáng)剛之手，并停留在我的嘴唇與野生親和力。。。。
7. Similarly, the man is a male symbol of conquest, a master, like the nearby Guangdong Danxiashan Yang Yuan Shiyi stand between heaven and earth, life itself is a tension masculine declaration is a forceful conquest of nature display. 同樣，男人的雄性象征是一種征服、一種駕馭，就象廣東丹霞山附近的陽(yáng)元石屹立于天地間，本身就是一種生命的張力，陽(yáng)剛的宣言，是一種征服自然界的有力展示。
8. This topic through in improvement sputamentum processing and examination some steps and the method, attempts, in does not lengthen the examination time in the situation to enhance the clinical smear the masculine detection rate, and reduces as a result of processes improper causes the bacterium death the negative rate. 本課題通過(guò)改良痰液處理和檢測(cè)中的一些步驟和方法，試圖在不延長(zhǎng)檢測(cè)時(shí)間的情況下提高臨床涂片的陽(yáng)性檢出率，并減少由于處理不當(dāng)而造成細(xì)菌死亡的陰性率。
9. However, teachers of the department have worried that too many male applicants are womanlike and asked them to try and be more masculine. 該系的老師擔(dān)憂許多男考生太奶油，提醒他們多一些陽(yáng)剛之氣。
10. I'm just a normal boy That sank when I fell overboard My ship would leave the country But I'd rather swim ashore Without a life that's sadly stuck again Wish I was much more masculine Maybe then I could learn to swim Like'fourteen miles away'You're floating up and down I spin, colliding into sound Like whales beneath me diving down I'm sinking to the bottom of my Everything that freaks me out The lighthouse beam has just run out I'm cold as cold as cold can be be I want to swim away but don't know how Sometimes it feels just like I'm falling in the ocean Let the waves up take me down Let the hurricane set in motion Let the rain of what I feel right now...come down Let the rain come down Where is the coastguard I keep looking each direction For a spotlight, give me something I need something for protection Maybe flotsam junk will do just fine The jets, I'm sunk, I'm left behind I'm treading for my life believe me (How can I keep up this breathing) Not knowing how to think I scream aloud, begin to sink My legs and arms are broken down With envy for the solid ground I'm reaching for the life within me How can one man stop his ending I thought of just your face Relaxed, and floated into space I want to swim away but don't know how Sometimes it feels just like I'm falling in the ocean Let the waves up take me down Let the hurricane set in motion Let the rain of what I feel right now...come down Let the rain come down Let the rain come down Now waking to the sun I calculate what I had done Like jumping from the bow Just to prove I knew how It's midnight's late reminder of The loss of her, the one I love My will to quickly end it all So thought no end my need to fall Into the ocean, end it all Into the ocean, end it all Into the ocean, end it all into the ocean...end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all I want to swim away but don't know how Sometimes it feels just like I'm falling in the ocean Let the waves up take me down Let the hurricane set in motion Let the rain of what I feel right now...come down Let the rain come down Let the rain come down Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all Into the ocean end it all goodbye 我只是一個(gè)正常的男孩這沉沒(méi)當(dāng)我落水我國(guó)船舶將離開(kāi)該國(guó)但我寧愿游泳上岸沒(méi)有一個(gè)生命的不幸再次陷入希望我更是男性化也許我可以學(xué)會(huì)游泳像'一十四英里以外'你是浮動(dòng)向上和向下余旋轉(zhuǎn)，碰撞成聲音像鯨潛水箱底下下跌我沉到水底，我一切怪胎我出去燈塔的光束剛剛用完我冷冷可冷是我要游泳遙遠(yuǎn)，但不知道如何有時(shí)感覺(jué)就像我屬于大海讓海浪把我跌讓颶風(fēng)啟動(dòng)讓雨什么我覺(jué)得現(xiàn)在來(lái)了。。。讓雨水下降哪里是海岸警衛(wèi)隊(duì)我一直期待每個(gè)方向?qū)τ谝粋�(gè)聚光燈，給我的東西我需要的東西保護(hù)也許廢料垃圾將做到罰款該飛機(jī)，我沉沒(méi)，我留下我踩我相信我的生活（我要如何保持這樣的呼吸）不知道如何思考俺大聲尖叫，開(kāi)始下沉我的腿和胳膊細(xì)分隨著羨慕的堅(jiān)實(shí)基礎(chǔ)我很深遠(yuǎn)的生命在我如何才能一人停止其結(jié)束我以為只有你的臉?lè)潘�，漂流進(jìn)入太空我要游泳遙遠(yuǎn)，但不知道如何有時(shí)感覺(jué)就像我屬于大海讓海浪把我跌讓颶風(fēng)啟動(dòng)讓雨什么我覺(jué)得現(xiàn)在來(lái)了。。。讓雨水下降讓雨水下降現(xiàn)在醒來(lái)太陽(yáng)本人計(jì)算，我做了就像跳弓只是為了證明我知道如何現(xiàn)在是午夜已故提醒失去她，一個(gè)我愛(ài)我將盡快結(jié)束這一切因此，認(rèn)為沒(méi)有必要為此，我國(guó)將下降入海，結(jié)束這一切入海，結(jié)束這一切入海，結(jié)束這一切到大洋。。。這一切結(jié)束入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切我要游泳遙遠(yuǎn)，但不知道如何有時(shí)感覺(jué)就像我屬于大海讓海浪把我跌讓颶風(fēng)啟動(dòng)讓雨什么我覺(jué)得現(xiàn)在來(lái)了。。。讓雨水下降讓雨水下降入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切入海結(jié)束這一切
11. Be engaged in clinical over 20 years, and being good at disposing common ailment and severe cases in anorectum section, masculine branch and urinary surgery, especially in operation on internal pile, external pile, combined hemorrhoid, anal fissure, colorectal Polyp, pruritus ani, rectocele, prolapse of ring-like hemorrhoids. 從事臨床工作二十余年，能嫻熟處理泌尿外科、男性科、肛腸外科各種常見(jiàn)病及疑難雜癥，尤其擅長(zhǎng)診治內(nèi)痔、外痔、混合痔、肛裂、直腸息肉、肛門瘙癢、脫肛、混合痔環(huán)狀脫出及內(nèi)痔環(huán)狀脫出，備受患者好評(píng)。
12. Methods The CHF animal models were established by the abdomen arteriarctia method, and the rats were randomly divided into sham, model, high, lowdose Tingli Shengmai decoction and masculine medicine control groups. 采用腹主動(dòng)脈縮窄法復(fù)制CHF大鼠模型，隨機(jī)分為假手術(shù)組、模型組、葶藶生脈方高、低劑量組、陽(yáng)性藥心寶丸對(duì)照組。
13. Eleanor had grown more conservative and didn't like seeing Hicky slopping around in masculine pants and old sweater. 埃莉諾變得更加保守，不喜歡看見(jiàn)希姬穿著男式褲子和舊毛衣到處跑。
14. Through to 1 example 82 year old of old age masculine spontaneity aeropleura nursing, sincerely feels the importance which the psychology nurses. 通過(guò)對(duì)1例82歲老年男性自發(fā)性氣胸的護(hù)理，深切感受到心理護(hù)理的重要性。
masculine什么意思
15. The local press says that more tradition-minded locals are upset by the growing number of young women affecting a masculine style of dress, baggy trousers, short hair and deep voices. 越來(lái)越多的年輕女性熱衷穿著男性化風(fēng)格的女裝，寬松的褲子，留短發(fā)并用低沉的聲音說(shuō)話，當(dāng)?shù)孛襟w稱那些思想更為傳統(tǒng)的當(dāng)?shù)鼐用駥?duì)此十分心煩。
16. Methods:1、Fluorescent quantitation PCR ex-reference strain and standard curve: Design of the primer, determination of the reaction system, chemical synthesis of masculine template, PCR amplification and identification, extraction and depuration of the DNA fragment, combinational joining with P carrier, convertion into the Bacillus coli DH5αcompetent cell, masculine clone appreciation of the microbial population, extraction of the recombinant plasmid and gradient deliquation, PCR reaction and description of standard curve; 2、We chose 113 people who received transplantation operation in 2005.1 to 2009.2 as the research objects. We made the examination of urinary castoff cells, obtained the peripheral serum and urine sample, extracted the serum and urinary DNA and took the message of the condition of the BKV infection by RT-PCR reaction. We divided the objects in three groups: Urine and Serum, Urine but Serum, Urine and Serum by the result of PCR detection. All of the examination data was analyzed statistically. 1、實(shí)時(shí)熒光定量PCR外參照系和標(biāo)準(zhǔn)曲線的合成：查閱文獻(xiàn)并設(shè)計(jì)反應(yīng)引物，確定反應(yīng)體系，化學(xué)合成BK病毒陽(yáng)性模板，通過(guò)PCR擴(kuò)增鑒定、DNA片段的提取和純化、重組連接P載體、轉(zhuǎn)化入大腸桿菌DH5α感受態(tài)細(xì)胞中、進(jìn)行菌群的陽(yáng)性克隆鑒定、提取重組質(zhì)粒并進(jìn)行梯度稀釋，RT-PCR反應(yīng)繪制標(biāo)準(zhǔn)曲線；2、選擇2005年1月-2009年2月腎移植術(shù)受者113例作為受試對(duì)象，留取外周血和尿液標(biāo)本，行尿脫落細(xì)胞檢查并分別提取血樣DNA和尿樣DNA，進(jìn)行RT-PCR反應(yīng)，按照PCR檢測(cè)結(jié)果將試驗(yàn)對(duì)象分為BKV血尿雙陰性組、尿液?jiǎn)侮?yáng)性組、血尿雙陽(yáng)性組，明確試驗(yàn)對(duì)象的BKV感染情況，將試驗(yàn)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。
17. Moreover! Time masculine semination, because inhaled a moment ago the secretion, the seminal fluid is diluted, and has one? 而且！男性射精時(shí)候由于剛才吸入的分泌物，精液被稀釋，而且有一部？
18. This con ten is the syzygy motif, and it expresses the fact that a masculine, element is always paired with a feminine one. 這個(gè)內(nèi)容正是回合主題，并且它表達(dá)了這樣一個(gè)事實(shí)：陽(yáng)性的元素總是伴隨著一個(gè)陰性的因素。
19. As this piece of land excessively rough and masculine, from the beginning of that generation to the first Long before the birth of a woman no longer 由于這片土地過(guò)分地粗獷和陽(yáng)剛，從向先龍那一輩起就沒(méi)再誕生過(guò)女人
20. The motif of using the sheep to punish the jealous woman shows a strong tendency of masculine superiority culture. 母題利用變羊信仰教訓(xùn)妒婦，故事主流傾向被注入了男性強(qiáng)勢(shì)文化導(dǎo)向。
masculine 詞典解釋
1.
1. 男性的;男子的 Masculine qualities and things relate to or are considered typical of men, in contrast to women.
e.g. ...masculine characteristics like a husky voice and facial hair. 沙啞的嗓音和胡子拉碴的面孔之類的男性特征 e.g. ...masculine pride. 男性的驕傲
2. masculine是什么意思
2. 有男子氣概的;陽(yáng)剛的;男子漢的 If you say that someone or something is masculine, you mean that they have qualities such as strength or confidence which are considered typical of men.
e.g. ...her aggressive, masculine image... 她那咄咄逼人、男人般的形象 e.g. The Duke's study was very masculine, with deep red wall-covering and dark oak shelving. 公爵的書(shū)房頗具陽(yáng)剛氣，深紅色墻紙，深色的橡木擱板。
3. masculine的意思
3. (某些語(yǔ)言中的詞)陽(yáng)性的 In some languages, a masculine noun, pronoun, or adjective has a different form from a feminine or neuter one, or behaves in a different way.
masculine 單語(yǔ)例句
1. masculine
1. Mine is a sleek city runabout compared to the weighty, more masculine model that comes with a reinforced crossbar designed to carry pigs.
2. They say the male contestants do not reflect masculine qualities, cry too much and set poor examples for all the guys out there.
3. The study also found that men can use credit in one masculine domain to resist pressure to engage in other masculine behaviors.
4. The measures extend protections to men perceived as effeminate and women viewed as masculine.
5.
5. These years are believed to lack yang or masculine energy to balance the feminine yin.
6.
6. One explanation for women dressing like men is that women had to look masculine in public to exude an air of authority.
7. It makes extensive use of strong masculine materials like oak, nickel and bronze.
8.
8. No wonder many people say that compared to the older generation today's young men are less masculine and women less feminine.
9. Serious clothes are generally the order of the day for women at such conferences and most are likely to pick garments with a masculine cut.
10. The Flying Tourbillon combines the watchmaking traditions of Geneva with the modernity of masculine, elegant and graphical design.
